Lasithi prefecture hosts mainly small mammals such as Acomys minous (a small rat), the Cretan migalis (small mammal), weasel, zourida (beech marten) and arkalos (badger).
Out of the 412 bird species that live or pass by Crete, Eleonora's falcon, imperial eagle and booted eagle use Lasithi area as hunting place during winter or summer. In Cha’s gorge you can see the largest colony of chough in Crete. In the prefecture you can see all kinds of serpents (eleven altogether and all of them are harmless) and amphibians (three species) that exist in Crete.
The coasts of the prefecture constitute important biotopes for Caretta caretta sea turtle, while scientists have seen several times the Mediterranean seal Monachus monachus.